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2025/11/17
Narrative Review of Patient Cancer Tissue-Derived Zebrafish Xenograft Models for Evaluating Drug Sensitivity as an Avatar Model for Clinical Application

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Zebrafish Xenograft Model for Predicting Cisplatin Efficacy in Muscle-Invasive Bladder Cancer

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AI-Driven Image Analysis for Precision Screening Transposon-Mediated Transgenesis of NFƒΘB eGFP Reporter System in Zebrafish

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Variation and classification of chemically-induced zebrafish malformations for the ICH S5 (R3) guideline: an atlas for zebrafish teratogenesis

2024/08/06
Validation of a new protocol for a zebrafish MEFL (malformation or embryo-fetal lethality) test method that conforms to the ICH S5 (R3) guideline

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tMyosin Light Chain Kinase Occurs in Bullfrog Sympathetic Neurons and May Modulate Voltage-Dependent Potassium Currents.

                     
1993/12/11

Akasu T, Ito M, Nakano T, Schneider CR, Simmons MA, Tanaka T, Tokimasa T, Yoshida M.
Neuron. 1993 Dec;11(6):1133-45.

Abstract

A polyclonal antibody against myosin light chain kinase (MLCK) of chicken gizzard recognized a 130 kd peptide of bullfrog sympathetic ganglia as MLCK. MLCK immunoreactivity was confined to the neuronal cell body. A synthetic peptide corresponding to an inhibitory domain of MLCK (Ala783-Gly804) was applied intracellularly to isolated sympathetic neurons during whole-cell recordings of ionic currents. The peptide inhibitor reversibly decreased M-type potassium current (IM) while not affecting A-type of delayed rectifier-type potassium currents. Intracellular application of an active fragment of MLCK enhanced IM, whereas application of an inactive MLCK fragment did not. The results suggest that IM can be modulated by MLCK-catalyzed phosphorylation.

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Pubmed